FastQCFastQC Report
Tue 1 Mar 2016
K27m3_WNN3_7725_GTCCGC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK27m3_WNN3_7725_GTCCGC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46302250
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT33900.031.27921544
TCTCGTA33950.031.0391243
TATTCGT26350.030.9151341
TAAGCCG44200.026.92686716
CCGATGA45250.026.49602920
ACGTCCG40750.026.18347732
TTCGGTA36150.025.9241325
AGCCGAT46850.025.87288518
TCGGTAT36500.025.73581126
CGGTATT37150.025.46317127
CGTCCGC41900.025.04542233
CGATGAC49500.024.31000321
ATTCGGT40850.023.47995824
AATTCGG40800.023.29305523
ATCTCGT46550.023.01610442
CGCACAT48100.022.5044537
GAATTCG43200.021.99899522
GATCGGA51400.021.5025831
ATCGGAA52200.021.1983342
GATGACG57750.020.7990522
Produced by FastQC (version 0.11.2)