FastQCFastQC Report
Tue 1 Mar 2016
K27m3_WNN1_7711_ATCACG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK27m3_WNN1_7711_ATCACG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42791726
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG54200.032.83669343
CTCGTAT54150.032.82876642
TCTCGTA54900.032.3820641
CGTATGC57350.031.18618444
ATCTCGT68100.026.52537540
CGATCTC72100.024.87048538
ACGATCT73850.024.31093237
TCACGAT75350.023.88481335
CACGATC77250.023.3547836
GATCTCG78500.023.09524339
ATCACGA82600.021.76035134
TATTCGT14400.021.2532061
CATCACG87700.020.46909733
GATCGGA95750.019.2928031
ATCGGAA97450.018.9394762
CGTCTGA98450.018.63659516
ACGTCTG107100.017.50124515
ACACGTC106650.017.32872813
TAAGCCG32850.016.67555216
CCGATGA32200.016.46451820
Produced by FastQC (version 0.11.2)