FastQCFastQC Report
Tue 1 Mar 2016
K27m3_WEN3_7718_GTTTCG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK27m3_WEN3_7718_GTTTCG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44527167
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT50650.033.70395744
CGTTTCG50750.033.3376333
TCTCGTA53350.031.99894543
TCGGAAT57150.029.60647637
TATTCGT26400.029.0217591
TTTCGGA61500.027.54813835
AATCTCG65100.026.19120241
CGGAATC64950.025.88126438
TAAGCCG48000.025.84874216
ATCTCGT66300.025.71585342
CGTCATA48450.025.29045327
GTTTCGG68500.024.89357834
TTCGGAA69250.024.46513436
TTCGGTA38300.023.89386225
CCGATGA51350.023.81914120
TCGGTAT38450.023.74343326
AGCCGAT51900.023.65149518
ATCGGAA74500.023.6225072
CGGTATT38950.023.49530427
GATCGGA75400.023.3596881
Produced by FastQC (version 0.11.2)