FastQCFastQC Report
Tue 1 Mar 2016
K27m3_WEN1_7724_CTTGTA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK27m3_WEN1_7724_CTTGTA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45579653
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA734600.16116840556026174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGT52650.036.2985881
TCGTATG48400.034.18096543
CGTCATA96250.033.82668727
CTCGTAT49000.033.76331342
CCGATGA98600.033.06509420
AGCCGAT98850.032.98147218
TAAGCCG98600.032.79822516
CGATGAC100850.032.6327921
TCTCGTA50700.032.63228241
ATGACGT102200.032.05105623
ACGTCAT101800.031.96089626
CGTATGC53300.031.07949344
GATGACG106350.030.80035422
TCATAAC109950.029.7318629
TCGGTAT77800.029.20917126
TTCGGTA77800.029.09606725
CGGTATT80550.028.32120527
ATAACAT116950.027.8963631
GTCATAA119300.027.4754228
ATTCGGT83650.027.24534424
Produced by FastQC (version 0.11.2)