FastQCFastQC Report
Tue 1 Mar 2016
K27ac_WNN3_7617_GGCTAC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK27ac_WNN3_7617_GGCTAC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41036413
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC1648560.4017310187418184TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT187550.040.01521342
TCGTATG188050.039.95221343
TCTCGTA194700.038.64239541
CGTATGC197950.037.95261844
ACGGCTA216550.034.99814232
ATCTCGT219350.034.37008340
TACATCT226100.033.41233437
CGTCTGA238850.032.95642516
GCTACAT230200.032.8631935
ACACGTC245050.032.32948313
AGTCACG235100.032.20548228
ACGTCTG246550.032.0702915
CACGTCT255600.030.96924414
CTACATC245800.030.80607436
CACACGT261150.030.4037512
ATCGGAA264250.030.2298722
ACATCTC254750.029.79288738
CATCTCG263600.028.82602339
GTCTGAA276850.028.5742517
TCTGAAC277800.028.37154618
Produced by FastQC (version 0.11.2)