FastQCFastQC Report
Tue 1 Mar 2016
K27ac_WNN1_7616_GATCAG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK27ac_WNN1_7616_GATCAG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43389825
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC2286810.5270383090966603TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT255450.041.44613642
TCGTATG255950.041.39621743
TCTCGTA259200.040.82655341
CGTATGC261000.040.6104844
ATCTCGT271500.039.03373340
ACGATCA273650.038.6768632
TCACGAT281050.037.68686330
CACGATC289850.036.55194531
CGATCAG291850.036.30413433
GTCACGA293600.036.0978229
GATCTCG297400.035.693839
ATCGGAA309650.035.1268922
ACACGTC311000.034.88216413
TATTCGT33600.034.6431051
CGTCTGA313450.034.54547516
AGTCACG312500.034.0483728
GATCGGA320000.034.0304641
ATCAGAT312900.033.93072535
ACGTCTG320050.033.8817515
CACGTCT334350.032.42635714
Produced by FastQC (version 0.11.2)