FastQCFastQC Report
Tue 1 Mar 2016
K27ac_WEN3_7625_ATGTCA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK27ac_WEN3_7625_ATGTCA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43393616
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT1376490.31721025507530876TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT158050.039.9475544
TCTCGTA161500.039.10888743
ATCTCGT174800.036.2351942
AATCTCG197350.032.27325441
CGTCTGA203900.031.9084716
ACACGTC208650.031.22463413
ACGTCTG215600.030.22829815
ATCGGAA217800.030.113552
GATCGGA223650.029.3685381
CACGTCT229550.028.44880314
TCGGAAG238400.027.5576023
CACACGT238050.027.51591912
ACATGTC241100.026.47881132
CGGAAGA249750.026.3842454
GAATCTC251800.025.27705840
GTCACAT258450.024.76064929
GCACACG269900.024.41511511
TCTGAAC273150.023.96034618
CATGTCA269300.023.8367633
ATGTCAG278100.023.09130334
Produced by FastQC (version 0.11.2)