FastQCFastQC Report
Tue 1 Mar 2016
K27ac_WEN1_7628_CAGATC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameK27ac_WEN1_7628_CAGATC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37719038
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1493760.39602282539655437TruSeq Adapter, Index 7 (100% over 50bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA921530.24431429030613133No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCAT521070.13814509267176964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG164550.040.4726143
CTCGTAT165100.040.3569442
TATTCGT62250.039.3387831
TCTCGTA170750.039.00955641
CGTATGC170850.038.97493444
ATCTCGT178400.037.4231240
CGTCATA115350.035.03285227
ATCGGAA201050.034.897082
ACACGTC205700.033.9472313
CGTCTGA206200.033.82174316
GATCGGA211200.033.3157581
GATCATC204800.032.74803536
CATCTCG207000.032.36951439
ACGTCTG215600.032.30679715
AGATCAT210250.031.86239635
ATGACGT128900.031.46965223
ACGTCAT129600.031.4015226
CACGTCT224250.031.16854314
TCATAAC131200.030.96856529
TATAAGC130900.030.9586314
Produced by FastQC (version 0.11.2)