FastQCFastQC Report
Tue 1 Mar 2016
CTCF_WNN3_8278_CAGATC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTCF_WNN3_8278_CAGATC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38780889
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1722340.4441208142495135TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT197800.040.189942
TCTCGTA197600.040.11880541
TCGTATG202650.039.30408543
CGTATGC205900.038.68369344
ATCGGAA231000.037.133052
GATCGGA231700.037.0397831
ATCTCGT215650.036.8912240
ACACGTC233800.036.30769313
CGTCTGA234850.036.05541616
ACGTCTG241800.035.1009715
GATCATC228900.034.67702536
CATCTCG232550.034.26553339
AGATCAT237900.033.4800135
CACGTCT254200.033.41443314
TCGGAAG261100.033.023453
CACACGT260850.032.70974712
CGGAAGA279750.030.7497314
ATCATCT259500.030.67527837
ACCAGAT275850.029.19906632
CAGATCA291250.027.57851434
Produced by FastQC (version 0.11.2)