FastQCFastQC Report
Tue 1 Mar 2016
CTCF_WEN3_8280_ATTCCT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTCF_WEN3_8280_ATTCCT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41013564
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT1612590.39318455718698336TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT186200.039.9282144
TCTCGTA188650.039.47968743
TATCTCG190450.038.97901541
GATCGGA216600.037.4183771
ATCGGAA218050.037.150062
CGTCTGA222450.036.0874216
ATCTCGT206850.035.99540742
ACACGTC224750.035.7541313
ACGTCTG229800.034.9236115
CACGTCT241050.033.3940114
TCGGAAG245400.033.093143
CGGAAGA261150.031.0446284
CACACGT259800.031.0218112
TTATCTC251400.029.72790740
CTTTATC256200.029.19070238
CCTTTAT262250.028.48183637
TTTATCT271400.027.64092339
GTCACAT274450.027.52863529
GCACACG299200.027.05253211
TCACATT279950.026.9092330
Produced by FastQC (version 0.11.2)