FastQCFastQC Report
Tue 1 Mar 2016
CTCF_WEN1_8282_AGTCAA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTCF_WEN1_8282_AGTCAA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39232341
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1736280.44256344529631814TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT203450.040.31081444
TCTCGTA205050.040.0714343
AATCTCG214850.038.40667341
GATCGGA229400.038.2053341
ATCGGAA233100.037.6458852
ATCTCGT220850.037.2141942
CGTCTGA241250.036.31792416
ACACGTC242150.036.2358813
ACGTCTG248750.035.29365515
CACGTCT259700.033.8819414
TCGGAAG262350.033.5190853
AACAATC249750.033.14010238
CACACGT273300.032.11312
CGGAAGA274050.032.0714344
ACAATCT258600.032.069839
AGTCAAC263650.031.48891434
TCAACAA274600.030.22074336
GTCAACA276050.030.08959235
CAACAAT282100.029.52057637
CAATCTC283800.029.25408740
Produced by FastQC (version 0.11.2)