FastQCFastQC Report
Tue 1 Mar 2016
79me2_WNN1_6760_TTAGGC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename79me2_WNN1_6760_TTAGGC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42449518
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT36750.028.85338442
TCGTATG37050.028.50096743
TCTCGTA37400.028.3519241
CGTATGC41350.025.59031344
ATCTCGT50700.021.1313940
GATCGGA55250.019.5367071
ATCGGAA57500.018.8999232
CATCTCG61700.017.86321439
CGTCTGA68700.015.81862116
ACACGTC70000.015.65250713
CGTCATA22000.014.4991727
ACGTCTG76400.014.33949415
TCGGAAG85850.012.9661523
CACGTCT88100.012.38552414
TTCGGTA16000.012.23680125
CGGAAGA93750.011.9907814
TATTCGT13850.011.9288621
TAAGCCG27800.011.63244216
TCGGTAT16300.011.60669826
AGCCGAT28150.011.48781118
Produced by FastQC (version 0.11.2)