FastQCFastQC Report
Tue 1 Mar 2016
79me2_WEN2_6765_ATGTCA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename79me2_WEN2_6765_ATGTCA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32924130
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT379270.1151951471458775TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT48800.035.7480444
TCTCGTA50650.034.44254343
AATCTCG56950.030.55527741
ATCTCGT63800.027.378142
CGTCTGA88550.020.49617416
TATTCGT12550.020.3628941
GATCGGA92300.019.4766221
ATCGGAA93000.019.3968432
ACACGTC95050.019.21152313
ACGTCTG98200.018.59405115
CGTCATA22350.018.3077227
GAATCTC101450.017.5862140
CGGTATT16150.017.5717927
TTCGGTA16800.017.28476525
AATTCGG16950.017.13180423
TCGGTAT16850.016.84180626
CACGTCT109300.016.82618314
TCGGAAG113750.015.9165373
CGGAAGA115800.015.6157714
TAAGCCG26450.015.30380916
Produced by FastQC (version 0.11.2)