FastQCFastQC Report
Tue 1 Mar 2016
79me2_WEN1_6751_ATCACG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename79me2_WEN1_6751_ATCACG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44039085
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT56750.035.31432742
TCGTATG58800.034.0457243
TCTCGTA59650.033.63433541
CGTATGC61850.032.50872844
CGTCATA51300.028.98830627
TATTCGT21900.028.3692191
ACGATCT70850.028.16215337
ATCACGA71650.027.81669434
ATCTCGT73600.027.43868640
TCACGAT74950.026.70934535
CGATCTC76050.026.35224538
CACGATC76250.026.25403436
ATCGGAA78750.025.9798852
GATCGGA79400.025.8605521
ACGTCAT60000.025.0416526
GATCTCG83300.024.32277339
AGCCGAT63100.024.22988918
CCGATGA62250.024.20734620
ATGACGT62550.024.02076723
CGATGAC63750.023.91382221
Produced by FastQC (version 0.11.2)