FastQCFastQC Report
Fri 19 Feb 2016
WNN2_ATAC_D_6009_CGTACTA_read2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWNN2_ATAC_D_6009_CGTACTA_read2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10924656
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG5800.039.7280651
GTCCTAC18950.028.8788431
CTGTAGG18850.026.7400461
GAGTACG3909.229227E-625.848711
GTACTAG7359.094947E-1225.4719071
AGTACGA4601.1699194E-625.0473372
TAGGACG16900.024.7174914
GCCTATG6852.9485818E-923.1263731
GTCCTAA19950.023.1000291
CCTACAG25650.021.8044363
GTAGGAC24200.021.666473
TCCGCGA4350.021.2996398
GGACGTG17650.021.1992686
TGACGGC12150.020.9367132
TACAGTG27750.020.6985035
GACGTGG11000.020.0857097
TTGACGG13050.019.88033531
CTACAGT27000.019.6527564
ATACGCG4105.2750693E-1119.2754044
ACGCGTA3900.019.1880556
Produced by FastQC (version 0.11.2)