FastQCFastQC Report
Fri 19 Feb 2016
WNN2_ATAC_D_6009_CGTACTA_read1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWNN2_ATAC_D_6009_CGTACTA_read1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10924656
Sequences flagged as poor quality0
Sequence length50
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAA20950.025.28329733
GTCCGCA21250.024.42095632
CCGCAAA23400.023.60875934
ATCCCGC6450.022.68901443
AGTCCGC24150.021.66767331
TAGTCCG24500.021.43848430
GCGATCG900.003528082320.84674343
CGTTGTA17850.020.12018635
TATACCG14650.020.070422
GATCCCG7100.020.02159142
TTCGCGG1508.214973E-620.01287343
GCGCGAT1609.165571E-619.76079644
ATCGTCG1651.3785047E-619.3845442
TCGTCGA1552.4709334E-718.77177639
CGCCTAG7150.018.70071618
ACGTCGC1151.2430514E-418.45553840
ATACCGC16400.018.3056413
CGACGTC1300.001016182718.24073644
CGCACCT30600.017.941798
CGCAAAA24150.017.79098935
Produced by FastQC (version 0.11.2)