FastQCFastQC Report
Fri 19 Feb 2016
WNN2_ATAC_C_6008_TAAGGCG_read2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWNN2_ATAC_C_6008_TAAGGCG_read2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8565663
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG3101.9692015E-742.2970471
GATCACG2450.004683344630.582121
CTGTAGG17250.029.3189471
CGCGACG906.228061E-428.0782363
GCGTCAC2750.00823407827.2458881
GTAGGAC16250.025.1926573
TTATACG3750.001232759824.9753992
GCGTAGA5301.2954226E-524.7397821
ATACGCG3201.8189894E-1224.334864
TGTAGGA21000.024.0834222
TACGCGT3650.024.076645
TAGGACG14300.023.72714
TAGGACC9650.021.3267784
CACGAGA6402.0372681E-1020.487631
GGACGTG14500.019.5886216
CGAACGG4600.019.05722830
ACGCGTA4550.018.9646386
CGCGTAT4750.018.9233887
GCGTAAT4951.4551915E-1118.9207571
GTGCTAA5050.006929925618.5460871
Produced by FastQC (version 0.11.2)