FastQCFastQC Report
Fri 19 Feb 2016
WNN2_6637_TCCTGAG_read2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWNN2_6637_TCCTGAG_read2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40004807
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG81050.020.8017944
CGTCTAG38250.019.2240851
GGACGTG84850.019.112446
CTAGACG34000.016.6375393
GACGCAC34550.016.3039346
GACGTGG54650.016.1854577
AACTGCG51350.015.725877
TAGACGC66800.015.6839554
GACGTGA48500.015.6518777
TGCGACC49500.015.4690910
CTACGGT35550.015.34953313
CGTAATC29900.015.15561722
ACGCACC35900.015.1388047
GCGACCA50550.015.06007511
CTGCGAC53650.014.8468369
CCTACGG37250.014.82592712
TACGCCG20200.014.5999665
TACGGTG37250.014.5899514
CGACCAA52300.014.47208712
TTGACGG50350.014.46162131
Produced by FastQC (version 0.11.2)