FastQCFastQC Report
Fri 19 Feb 2016
WNN2_6637_TCCTGAG_read1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWNN2_6637_TCCTGAG_read1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40004807
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG45600.022.4414791
TAGGACG79550.021.3778884
GGACGTG82850.019.7831386
GACGCAC41850.018.7674036
CTAGACG42100.018.5523223
CTACGGT42200.018.03762413
ACGCACC43450.017.5697547
TACGGTG43900.017.43935614
CGTAATC31550.017.15351322
CGCACCT44900.017.050638
TAGACGC78100.016.9015184
TTGGACG33300.016.78063617
GACGTGA44300.016.736027
CCTACGG46400.016.49942412
TGGACGT34250.016.37942118
GACGTGG56050.016.2891527
TGACGGC62050.016.20342832
ACGGTGA48200.016.11185315
TTGACGG62400.015.97149631
CGGTCTA20800.015.75906610
Produced by FastQC (version 0.11.2)