FastQCFastQC Report
Fri 19 Feb 2016
WNN1_6640_AGGCAGA_read2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWNN1_6640_AGGCAGA_read2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37302951
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG118050.019.9525644
GGACGTG117750.018.7980426
CGTCTAG34800.017.781411
CTAGACG31800.016.8215243
GACGTGG75000.016.311567
CTACGGT31200.016.21863713
GACGCAC31750.016.0082476
TACGGTG32000.015.88190414
GACGTGA64350.015.5918727
CCTACGG32950.015.29103712
TAGACGC58050.015.2442054
GTAGGAC173750.015.1654583
ACGCACC33450.015.0633427
CGTAATC25150.014.95607422
CGCACCT34050.014.6688468
CTGTAGG180800.014.53051951
AGGACGT176400.014.4125185
TCTCGCC65150.014.314280543
ACGTGGA85900.014.241938
TTGGACG26650.014.19705617
Produced by FastQC (version 0.11.2)