FastQCFastQC Report
Fri 19 Feb 2016
WNN1_6640_AGGCAGA_read1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWNN1_6640_AGGCAGA_read1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37302951
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG39550.021.250811
TAGGACG122250.021.1432024
GGACGTG123000.020.0127226
CTAGACG34200.018.6532153
GACGTGA61900.018.0532047
CTACGGT35150.017.96074513
GACGCAC35100.017.8615426
TAGACGC65500.017.2289124
ACGCACC36400.017.1027627
TACGGTG36700.017.08230814
GACGTGG80800.016.634557
TCCTCGC79900.016.43852644
CCTACGG38300.016.36868912
TCTCGCC66850.016.19192343
TTGACGG50750.016.04174831
GTAGGAC182700.016.0258583
TCCGCAA39200.015.88481933
CGCACCT38800.015.8746668
TGACGGC52900.015.43165232
TTTCTCG70650.015.38288841
Produced by FastQC (version 0.11.2)