FastQCFastQC Report
Fri 19 Feb 2016
WNN1_6433_140424_read2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWNN1_6433_140424_read2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193080414
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG167700.018.5773281
TAGGACG335050.018.2645784
GGACGTG350300.016.4035976
CGTAATC138750.014.8179322
CTAGACG140500.014.1110373
TTGGACG149900.014.04305617
TAGACGC256600.013.9354634
TTGACGG225850.013.32807931
TGGACGT156350.013.28102718
GACGTGA205950.013.2503377
CTACGGT135750.013.19532913
GACGCAC144950.012.8099096
GACGTGG244150.012.7430577
TGACGGC238700.012.7031932
CCGTACG165000.012.66071533
CCGTAAC217100.012.539206529
ACGCACC147700.012.4214737
CGTACGT163500.012.3476434
TGCGACC188000.012.329555510
TACGGTG142600.012.32906114
Produced by FastQC (version 0.11.2)