FastQCFastQC Report
Fri 19 Feb 2016
WNN1_6433_140424_read1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWNN1_6433_140424_read1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193080414
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG182550.021.236241
TAGGACG350950.018.3979344
GGACGTG365400.016.5332666
TTGACGG241500.015.41861731
TGACGGC249550.015.04605132
CTAGACG164200.014.76769353
TAGACGC319000.014.6790944
CGTAATC112650.014.65784822
GACGGCT253050.014.61403333
CTACGGT165950.013.72593913
TACGGTG168050.013.51510314
GACGCAC174600.013.4347826
TTGGACG126100.013.3892617
GCTCGAA143400.013.36986511
TGCTCGA146400.013.27610910
ACGCACC175900.013.2230697
GACGTGA210700.013.167627
GACGTGG254150.013.0443877
TGGACGT132750.012.85101818
ACCGGTC97650.012.7652548
Produced by FastQC (version 0.11.2)