FastQCFastQC Report
Fri 19 Feb 2016
WNN1_500_6744_TCCTGAG_read2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWNN1_500_6744_TCCTGAG_read2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71709194
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG219350.019.79254
CGTCTAG69050.019.4221551
GGACGTG221300.018.7055326
GACGTGG142500.016.3435827
GACGTGA116700.015.9277997
CGTAATC54500.015.52062722
TTGGACG56050.015.48108417
TCCTCGC143650.015.25424944
TTTCGAC41150.015.14198336
CTAGACG55900.015.141853
TAGACGC107100.015.0037254
GACGCAC53600.015.0033026
GTAGGAC337100.014.9872283
CTACGGT55200.014.4857913
CTGTAGG349100.014.3902261
TGGACGT61050.014.35711418
AACTGCG83450.014.3491597
TACGGTG56350.014.22914714
GTCCTAC348200.014.092781
TTGACGG93000.014.05701931
Produced by FastQC (version 0.11.2)