FastQCFastQC Report
Fri 19 Feb 2016
WNN1_500_6744_TCCTGAG_read1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWNN1_500_6744_TCCTGAG_read1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71709194
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG73000.022.1190811
TAGGACG222600.020.7676014
GGACGTG225950.019.7099326
CTAGACG59050.017.393323
GACGTGA116750.017.2925197
GACGCAC59000.017.0347846
GACGTGG149300.016.660017
TCCTCGC147400.016.33920944
TAGACGC117900.016.2100664
CTACGGT61750.015.95772813
ACGCACC63450.015.7707467
CGTAATC50800.015.76027222
TGACGGC101100.015.53916332
TTGACGG101400.015.49115731
CGCACCT64800.015.4098078
TACGGTG63950.015.40875214
GACGGCT102050.015.39635233
GTAGGAC348850.015.3702333
CTGTAGG349600.015.3159191
CCTACGG66850.014.93772412
Produced by FastQC (version 0.11.2)