FastQCFastQC Report
Fri 19 Feb 2016
WEN2_6633_TCCTGAG_read2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWEN2_6633_TCCTGAG_read2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44422206
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG127350.020.391284
GGACGTG131200.018.862496
GACGTGG84700.015.895547
GACGTGA73700.015.7307847
CTAGACG32800.015.7124433
GTAGGAC190950.014.7751253
CGTCTAG38750.014.7340461
TCTCGCC75350.014.7117543
AGGACGT190600.014.5826075
TAGACGC61150.014.5517274
GACGCAC35400.014.2302496
CGACGAA31600.014.13035223
CTACGGT34250.014.06644613
TTTCTCG78850.014.03089341
TTTCGAC23350.013.94115536
CTGTAGG205400.013.7588321
ACGCACC35500.013.7572587
ACGTGGA98050.013.7312058
CGTGGAA97450.013.7039119
TCCTCGC87450.013.68216144
Produced by FastQC (version 0.11.2)