FastQCFastQC Report
Fri 19 Feb 2016
WEN2_6633_TCCTGAG_read1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWEN2_6633_TCCTGAG_read1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44422206
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG130450.020.253954
GGACGTG133850.018.9506976
CTAGACG38050.018.5598893
CGTCTAG40050.018.13261
GACGCAC41900.016.8015846
CTACGGT40500.016.73035213
TACGGTG41650.016.42686714
TAGACGC71250.016.2718354
GACGTGA69350.016.1784887
ACGCACC43000.016.1159677
GACGTGG90350.015.9487767
CCTACGG43900.015.68516712
CGCACCT45750.014.8106248
TCCGCAA46250.014.79375833
GTAGGAC204650.014.7921633
TCTCGCC72450.014.54539543
AGGACGT197700.014.3214045
TTTCTCG75350.014.24826741
GTCCTAC200700.014.2432941
ACGTGGA103150.014.0976278
Produced by FastQC (version 0.11.2)