FastQCFastQC Report
Fri 19 Feb 2016
WEN2_500_6743_AGGCAGA_read2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWEN2_500_6743_AGGCAGA_read2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84215767
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG220050.019.099014
GGACGTG221950.017.8273336
CGTCTAG78550.017.0443041
GACGTGG148700.015.2174347
TAGACGC112950.015.0041894
GACGTGA117600.014.9824297
CGTAATC61250.014.71014622
CGACGAA58350.014.6115423
CTAGACG57550.014.5923043
CTACGGT56800.014.30826613
TCCTCGC156500.014.22808744
CCGACGA59550.014.20526122
ACCGACG60050.014.18764521
AACTGCG99200.013.9965367
GACGCAC59150.013.8913566
TGCGACC95150.013.87403910
TTGGACG65900.013.86569217
TTTCGAC50400.013.80571636
GTAGGAC361500.013.7498543
TGGACGT68300.013.37846418
Produced by FastQC (version 0.11.2)