FastQCFastQC Report
Fri 19 Feb 2016
WEN2_500_6743_AGGCAGA_read1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWEN2_500_6743_AGGCAGA_read1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84215767
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG87000.021.1905671
TAGGACG231000.020.8789834
GGACGTG235300.019.3848766
GACGTGG159650.016.5994747
GACGTGA122100.016.2647847
TAGACGC133000.015.6596134
CTAGACG68900.015.6091143
TTGACGG113750.015.49156631
GACGCAC66500.015.4444146
TGACGGC115350.015.39299132
GACGGCT117050.015.20914533
CTACGGT67400.014.94635113
TCCTCGC156450.014.92978544
GTAGGAC385750.014.6697423
ACGCACC69200.014.6511277
CGGTCTA35600.014.58110210
CGTAATC63350.014.44357722
ACGTGGA183450.014.3873048
CTGTAGG395300.014.2305511
TACGGTG71300.014.159657514
Produced by FastQC (version 0.11.2)