FastQCFastQC Report
Fri 19 Feb 2016
WEN1_6634_TAAGGCG_read2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWEN1_6634_TAAGGCG_read2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48100041
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG170900.019.0214984
GGACGTG173000.017.7682386
CGTCTAG38100.017.7403531
CGTAATC28700.015.4833522
GACGTGA92100.015.4091857
GACGTGG110050.015.2150787
GTAGGAC231800.015.1071953
AGGACGT229150.014.9054975
TAGACGC54800.014.7799694
TCCTCGC116600.014.63774244
TCTCGCC89800.014.62205543
CTAGACG34200.014.416143
GACGCAC34050.014.2167266
GTCCTAC241300.014.2153961
TTTCTCG97900.013.97385841
TTCCTCG141050.013.41021843
CTGTAGG257050.013.3615191
CCGTACG33250.013.29824833
TTGGACG34450.013.28206317
TACGGTG35850.013.25656614
Produced by FastQC (version 0.11.2)