FastQCFastQC Report
Fri 19 Feb 2016
WEN1_6634_TAAGGCG_read1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWEN1_6634_TAAGGCG_read1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48100041
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG170250.020.0553284
GGACGTG175900.018.7483966
CGTCTAG41800.018.4265021
GACGTGG114950.016.6506927
GTAGGAC239250.015.7709773
AGGACGT237150.015.5666085
TAGACGC58500.015.53171354
GACGTGA91200.015.51094157
CTAGACG35800.015.4868783
TACGCCG15400.015.1429455
GACGCAC34950.014.7927546
TCCTCGC112450.014.71278544
ACGTGGA131200.014.5879078
CTACGGT36100.014.56507613
CGTAATC28250.014.48488822
TTGACGG53100.014.37677631
GTCCTAC250650.014.3724941
TCTCGCC86250.014.20775143
CTGTAGG265150.014.1425961
ACGCACC36750.014.0680067
Produced by FastQC (version 0.11.2)