FastQCFastQC Report
Tue 12 Aug 2014
5D_7_CAGATC_L005_R1_002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5D_7_CAGATC_L005_R1_002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Sequences flagged as poor quality0
Sequence length51
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG12100.010.9075223
CTCCGAT24350.09.6740931
CTCGTAT11700.09.40109342
TCCGATT20200.09.25826552
CGATTAG16700.09.2202614
CTCGGAT31350.09.1993941
CTCGGTT20650.09.1686171
CTCGTTT24700.09.0913571
CGGGAAT14950.08.541071
CCGATTA16600.08.4807083
TGCGCGA9950.08.4010929
CTGGAAT79450.08.3960341
CCGGTAT11550.08.3867981
TCGGTTT20300.08.3455842
CGCGAGT9550.08.29226111
TTCGATA10150.08.23722
CCCGAAT18800.08.08011
CGGGGAT24300.08.0632331
CTCGTGT28100.07.99133541
GTCGGTT15500.07.9539311
Produced by FastQC (version 0.11.2)