FastQCFastQC Report
Mon 1 Dec 2014
2014-06-03-PyN6_7159_TCAT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-06-03-PyN6_7159_TCAT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47796025
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGT7788081.6294409420030223Illumina Paired End PCR Primer 2 (100% over 44bp)
TGAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG1301020.2722025524089085Illumina Paired End PCR Primer 2 (100% over 43bp)
GAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTC994990.2081742153243915Illumina Paired End PCR Primer 2 (100% over 46bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT625770.13092511354239186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1014000.033.3824924
AAGATCG1031050.033.0533182
AGATCGG1034750.032.8674933
ATCGGAA1045650.032.403675
TCGGAAG1071800.031.6874376
ATCTCGT1068000.031.4038840
TATGCCG144100.029.98882938
CGGAAGA1151950.029.617187
CGTATGC148000.029.17165236
TCGTATG148850.028.97110235
GAAGATC1183750.028.7652151
ATGCCGT152650.028.3484739
GACCGAT1191350.028.06119235
ACCGATC1192350.028.01643436
AGCGGTT1222950.027.85962913
AATGCCG1218400.027.85109327
CGGTTCA1221800.027.77590415
CCGATCT1203600.027.74118437
GAGCGGT1231050.027.7344612
GATCTCG1220000.027.62728139
Produced by FastQC (version 0.11.2)