FastQCFastQC Report
Mon 1 Dec 2014
2014-06-03-MP5c_7155_CAGT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-06-03-MP5c_7155_CAGT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45161738
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT1254430.27776388942338753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC226200.027.84344912
CAACGCA230100.027.24907913
ATCAACG239300.026.34492711
AACGCAG252300.024.81916214
GGTATCA263550.024.2438328
ACGCAGA258550.024.15924515
GTGGTAT272250.023.4098156
TGGTATC274300.023.2353737
GTATCAA276550.023.1403399
CGCAGAG270200.023.03680216
GAGTACT239450.022.12411120
AGTGGTA291850.021.9876775
TATCAAC292800.021.83559210
AGAGTAC288550.020.95857819
AAGCAGT360150.018.8354361
GTACTTT284650.018.62367822
CAGAGTA337500.018.28026618
AGTACTT295550.017.9787421
GCAGTGG364800.017.8202673
CAGTGGT368750.017.607964
Produced by FastQC (version 0.11.2)