FastQCFastQC Report
Mon 1 Dec 2014
2014-06-03-M10c_7156_ATAT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-06-03-M10c_7156_ATAT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52944865
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGT45254538.547482366797988Illumina Paired End PCR Primer 2 (100% over 44bp)
TTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGT33777036.379661181495128Illumina Paired End PCR Primer 2 (100% over 44bp)
ATAGACGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA5025400.949176091014681Illumina Paired End PCR Primer 2 (100% over 41bp)
TATAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG2352010.444237604534453Illumina Paired End PCR Primer 2 (100% over 43bp)
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTAT2302910.4349638062161458Illumina Paired End PCR Primer 2 (100% over 46bp)
ATAGATGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA533370.10074064784186341Illumina Paired End PCR Primer 2 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGATC3681250.039.5264171
TAGATCG8758350.038.7375222
TAGACGG573800.038.240332
ATAGACG582450.037.9734731
ATAGATC5215950.037.676641
GATCGGA8972200.037.557794
AGATCGG9032900.037.5514873
ATCGGAA9043350.037.2569435
TCGGAAG9132200.036.971646
GACGGAA605950.035.7070054
ATCTCGT9413150.035.3610940
CGGAAGA9795550.034.573537
AGCGGTT9913400.034.2233513
GAGCGGT9940400.034.20109612
CGGTTCA9885400.034.19297815
AAGAGCG9953700.034.17709710
AGAGCGG9981750.034.1479511
GCGGTTC9920350.034.11566514
AATGCCG9888150.034.11423527
GACCGAT9759900.034.07834235
Produced by FastQC (version 0.11.2)