FastQCFastQC Report
Mon 1 Dec 2014
2014-06-03-F104_7153_TTGT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-06-03-F104_7153_TTGT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41662239
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT982380.23579625665341702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC199650.024.95544412
CAACGCA204200.024.3101213
ATCAACG211900.023.64564511
AACGCAG229150.021.78504614
GGTATCA233650.021.6373928
ACGCAGA233450.021.31718615
GTGGTAT241000.021.109916
GTATCAA243700.020.7614949
TGGTATC246050.020.5713317
AGTGGTA252450.020.2386255
GAGTACT208000.020.12343420
CGCAGAG251500.019.84341216
TATCAAC262900.019.24524910
AGAGTAC262550.018.64647119
AAGCAGT308250.017.2232281
GTACTTT252200.016.73006622
AGTACTT260250.016.3061321
CAGAGTA304850.016.2165918
CAGTGGT320750.016.0906374
GCAGTGG327450.015.8161573
Produced by FastQC (version 0.11.2)