FastQCFastQC Report
Mon 1 Dec 2014
2014-06-03-F103_7152_AACT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-06-03-F103_7152_AACT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42659842
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT2410750.565109922348048No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1547820.36282834802810565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC359000.031.56784212
CAACGCA365000.030.99851213
ATCAACG367800.030.90579411
GGTATCA388650.029.3508728
GTGGTAT392250.029.1672126
AACGCAG388350.029.13373414
GTATCAA395700.028.7571189
ACGCAGA392950.028.69300815
TGGTATC399100.028.5572977
AGTGGTA410300.028.0145975
TATCAAC415150.027.42428410
GAGTACT369150.027.42273520
CGCAGAG415400.027.09942416
AGAGTAC423350.025.86292319
AAGCAGT469450.025.5313871
GTACTTT411250.024.59941922
CAGTGGT480050.024.2684424
GCAGTGG491800.023.916073
AGTACTT425850.023.88892421
CAGAGTA464800.023.72431618
Produced by FastQC (version 0.11.2)