FastQCFastQC Report
Mon 1 Dec 2014
2014-06-03-BP5c_7160_GCTT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-06-03-BP5c_7160_GCTT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42465368
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG3903750.9192785047806484Illumina Paired End PCR Primer 2 (100% over 43bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT875530.20617506481987866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA453200.037.6333545
GACCGAT444900.037.45536
ACCGATC446650.037.21857537
AGCGGTT461550.036.9062214
AGATCGG466400.036.7046784
GATCTCG458600.036.67616340
CGGTTCA462750.036.67138716
AATGCCG462150.036.66385328
GAGCGGT467750.036.4607313
CCGATCT457550.036.34504338
CGAGACC464000.036.20865233
CGATCTC462500.036.08152839
GAGACCG464700.036.07232334
CAGATCG474650.036.0705833
AGACCGA465400.035.88536535
GCGGTTC474250.035.8660415
ATCGGAA476600.035.8042766
TGCCGAG475400.035.6546130
AGAGCGG482600.035.54363612
CCGAGAC477300.035.51833332
Produced by FastQC (version 0.11.2)