FastQCFastQC Report
Mon 1 Dec 2014
2014-06-03-B10c_7157_GAAT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-06-03-B10c_7157_GAAT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46227628
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGT28528336.171272728940364Illumina Paired End PCR Primer 2 (100% over 44bp)
TTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGT1211910.26216140702698393Illumina Paired End PCR Primer 2 (100% over 44bp)
TTCAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG746720.16153110862620942Illumina Paired End PCR Primer 2 (100% over 43bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT512810.11093149750188351No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT3226500.038.678740
GATCGGA3267900.038.6458174
AGATCGG3299500.038.5566523
CAGATCG3182750.038.4386252
ATCGGAA3310100.038.158225
TCGGAAG3352850.037.7230956
TCAGATC3317500.037.4382361
AGCGGTT3417750.037.12162413
CGGTTCA3409100.037.07110615
GACCGAT3364900.037.05395535
GAGCGGT3431950.037.0460812
AATGCCG3409750.037.0404927
ACCGATC3365750.036.9996436
CGGAAGA3428150.036.9958727
AAGAGCG3440950.036.96577510
CGAGACC3393450.036.92700232
AGAGCGG3452900.036.92583511
GATCTCG3396700.036.9120139
GAGACCG3389950.036.88369433
AGACCGA3388050.036.8647834
Produced by FastQC (version 0.11.2)