FastQCFastQC Report
Mon 1 Dec 2014
2014-06-03-8EF3_7154_ACGT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-06-03-8EF3_7154_ACGT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45974186
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT1194510.2598218922244757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG62700.028.3525922
TCAACGC232050.025.9185712
CAACGCA237850.025.26054613
ATCAACG242800.024.90328811
GGTATCA263250.023.185298
ACGCAGA258800.023.0932715
AACGCAG261650.022.99291614
CTCGTAT66600.022.24968340
GTGGTAT276200.022.1266966
GTATCAA277300.021.9816869
CGCAGAG275600.021.7005416
TGGTATC283850.021.4815147
GAGTACT237900.021.4192920
AGTGGTA286150.021.4054855
AGAGTAC293450.020.05663919
GATCGGA131500.020.0121574
TATCAAC313100.019.58964710
GTGATCG45650.019.1425252
ACCGATC137900.019.04058636
AAGCAGT345450.018.5582921
Produced by FastQC (version 0.11.2)