FastQCFastQC Report
Mon 1 Dec 2014
2014-03-26-PLP2_6534_CTTT_read2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-03-26-PLP2_6534_CTTT_read2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55709442
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG2588600.4646609097251414Illumina Single End PCR Primer 1 (100% over 42bp)
TAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT2289920.4110470178466336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC658500.035.24717313
ATCAACG666000.034.9411212
CAACGCA666600.034.85756714
GTATCAA684950.033.80467210
GCGTCGT356100.033.54680616
AACGCAG699200.033.37801415
GGTATCA696350.033.3000839
ACGCAGA702750.033.20673816
GATCGGA362750.033.0533526
GTCGTGT360700.033.03364618
TCGTGTA364300.032.7236819
CGTGTAG367600.032.5713920
TAAGCAG757050.032.5148851
ATCTCGG370500.032.48631340
CGTCGTG368200.032.47859617
AGAGTAC702100.032.45458620
GTGGTAT719800.032.30947
AGAGCGT373850.032.05064813
GATCTCG377600.031.8536939
TGGTATC729300.031.776158
Produced by FastQC (version 0.11.2)