FastQCFastQC Report
Mon 1 Dec 2014
2014-03-26-PLP2_6534_CTTT_read1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-03-26-PLP2_6534_CTTT_read1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55709442
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG2545110.4568543335975255Illumina Paired End PCR Primer 2 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG333700.033.1985540
GATCGGA371200.032.8018845
AATGCCG373100.031.92179528
AGCGGTT380800.031.80188414
GACCGAT364300.031.42798836
ACCGATC351750.031.38624437
GAGCGGT389450.031.1872713
CCGATCT354350.031.1237538
AGATCGG392500.031.108014
CGAGACC380500.030.97708933
CGGTTCA389650.030.96676616
ATCGGAA395700.030.7860016
GAGATCG399250.030.6465853
GAGACCG382950.030.63794734
AGACCGA386900.030.25788735
AAGAGCG404300.030.17059911
CGATCTC373150.029.63127339
CCGAGAC401250.029.46377832
GCGGTTC418300.028.95086315
TGCCGAG416000.028.77430330
Produced by FastQC (version 0.11.2)