FastQCFastQC Report
Mon 1 Dec 2014
2014-03-26-PLP1_6533_TTGT_read2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-03-26-PLP1_6533_TTGT_read2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58987961
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT3593630.6092141411702635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC941150.036.74453713
CAACGCA946450.036.59974714
ATCAACG949950.036.47842812
GTATCAA974000.035.3971810
ACGCAGA989400.035.2030716
AACGCAG990550.035.15813415
GGTATCA984850.035.0661059
TAAGCAG1058650.034.611541
AGAGTAC984750.034.3513920
GTGGTAT1009800.034.282427
TATCAAC1016650.033.91814411
AGTGGTA1024950.033.8067366
TGGTATC1023250.033.7263378
CGCAGAG1044950.033.37549217
CAGAGTA1030850.033.08289319
GAGTACT503000.031.93969221
AAGCAGT1100900.031.8795472
GCAGAGT1120200.030.84939618
CAGTGGT1140100.030.4844555
GCAGTGG1157800.030.2490714
Produced by FastQC (version 0.11.2)