FastQCFastQC Report
Mon 1 Dec 2014
2014-03-26-PLP1_6533_TTGT_read1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-03-26-PLP1_6533_TTGT_read1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58987961
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT799400.13551917822689277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC176200.024.1749912
CAACGCA188750.022.5569713
ATCAACG191050.022.42178211
GATCGGA70400.022.2222375
GTATCAA218900.019.7525629
AATGCCG80250.019.29463428
AACGCAG224100.019.13287514
ACGCAGA224900.018.90460215
GGTATCA229650.018.8279538
GACCGAT80550.018.6884336
ACCGATC78950.018.38759637
GAGTACT192050.018.26823820
AGCGGTT85850.018.22274814
CCGATCT81850.017.54087838
AGATCGG93200.017.1929244
AGAGTAC243850.017.1241919
GAGCGGT92250.017.0448313
TATCAAC255500.017.01635710
GTGGTAT256300.016.9631166
CGAGACC92950.016.79114533
Produced by FastQC (version 0.11.2)