FastQCFastQC Report
Mon 1 Dec 2014
2014-01-29_02-27_03-10-PyN5_5989_TGCT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-01-29_02-27_03-10-PyN5_5989_TGCT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6709084
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC15200.022.49531712
CAACGCA15200.022.36343413
ATCAACG17950.018.93784511
AACGCAG18650.018.54814114
ACGCAGA21050.016.43485815
GGTATCA21350.016.1092998
GTATCAA23050.015.1814489
CGCAGAG23450.014.9242716
TGGTATC23250.014.7097947
GAGTACT19000.014.6312620
GTGGTAT23000.014.6088136
AGAGTAC23900.014.30931119
TATCAAC25100.014.18052710
AGTGGTA26450.013.0056785
GTACTTT24800.011.6126522
CAGAGTA32350.010.880589518
AGTACTT26650.010.65642821
CAGTGGT35800.09.9998564
GCAGAGT36900.09.86391617
AAGCAGT38100.09.5017421
Produced by FastQC (version 0.11.2)