FastQCFastQC Report
Mon 1 Dec 2014
2014-01-29_02-27_03-10-PyN5_5989_TGCT_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-01-29_02-27_03-10-PyN5_5989_TGCT_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30183048
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC66050.022.24737512
CAACGCA71250.020.59568413
ATCAACG76150.019.42797511
AACGCAG81650.018.3643314
ACGCAGA92050.016.22511115
GGTATCA95150.015.6956388
GTATCAA95850.015.6227539
CGCAGAG99750.014.95309916
GAGTACT85150.014.42595320
TGGTATC103250.014.4071747
AGAGTAC104050.014.22090719
TATCAAC107200.014.15516210
GTGGTAT104800.014.0606156
CGAGCAA47700.013.91721418
AGTGGTA114900.013.1030475
TATGCCG17250.012.64006240
GATCGGA48200.012.03795725
CCGAGCA57400.011.80908117
GTACTTT109750.011.39308522
AAGATCG53200.011.16907223
Produced by FastQC (version 0.11.2)