FastQCFastQC Report
Mon 1 Dec 2014
2014-01-29_02-27_03-10-MP5b_6268_TATT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-01-29_02-27_03-10-MP5b_6268_TATT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47439327
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG14650653.088292125223446Illumina Paired End PCR Primer 2 (100% over 43bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT812450.17126086126812043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1618700.038.995365
AAGATCG1633500.038.7223783
CGGTTCA1621150.038.5575516
AGCGGTT1624950.038.5501314
ATCGGAA1636600.038.5456736
GAGCGGT1626400.038.53777313
AGATCGG1642500.038.4874654
AATGCCG1614800.038.4593228
GACCGAT1597650.038.42104736
GCGGTTC1633400.038.31574615
ACCGATC1602400.038.27055437
ATAAGAT1661050.038.1235851
AAGAGCG1649200.038.09201411
TAAGATC1661750.038.0810472
GATCTCG1613600.038.04764640
CGAGACC1621750.038.03986433
ATGCCGA1632750.038.03471829
GAGACCG1619750.038.0063934
CCGAGAC1630850.037.9742132
AGAGCGG1656050.037.9175712
Produced by FastQC (version 0.11.2)