FastQCFastQC Report
Mon 1 Dec 2014
2014-01-29_02-27_03-10-EP8b_6267_GAAT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-01-29_02-27_03-10-EP8b_6267_GAAT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58019144
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG908296015.655108596569436Illumina Paired End PCR Primer 2 (100% over 43bp)
TCAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGT720474712.41787882978763Illumina Paired End PCR Primer 2 (100% over 44bp)
TTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGT3747880.6459729912595746Illumina Paired End PCR Primer 2 (100% over 44bp)
TCAGACGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA768450.13244766244741565Illumina Paired End PCR Primer 2 (100% over 41bp)
TCAGATCCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG745020.1284093401998485Illumina Paired End PCR Primer 2 (100% over 39bp)
CAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA622430.1072801074073068Illumina Paired End PCR Primer 2 (100% over 45bp)
TCAGATTCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG598460.10314871243188282Illumina Paired End PCR Primer 2 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCAGAT10017800.039.0136951
ATCTCGT8498050.037.8982440
TAGATCG418050.037.8359682
TTAGATC479250.033.287671
TCTCGTA447050.031.4381440
GACGGAA123150.027.7423764
TCAGACG132450.025.2388171
CAGACGG132850.025.2231182
AGACGGA142300.024.0246263
GATCCGG149750.022.9345934
AGATTCG115950.022.5741863
ATTCGGA113150.022.5661935
ATCCGGA149250.022.1814615
TCAGTCG71600.021.9627511
CAGATCG17696050.021.9580883
ACGGAAG157150.021.9563395
TCAGATC17806250.021.8752772
AGATCCG167150.021.648293
GATCGGA18105350.021.482195
AGATCGG18100950.021.4563944
Produced by FastQC (version 0.11.2)