FastQCFastQC Report
Mon 1 Dec 2014
2014-01-29_02-27_03-10-E10h_6269_TCAT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-01-29_02-27_03-10-E10h_6269_TCAT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45436899
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGT6598761.452291011320997Illumina Paired End PCR Primer 2 (100% over 44bp)
TGAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG3358900.7392449911689616Illumina Paired End PCR Primer 2 (100% over 43bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT720770.15863098403788514No Hit
GAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTC614650.13527551693173426Illumina Paired End PCR Primer 2 (100% over 46bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT871450.032.33846340
CGTATGC97700.026.8658136
TATGCCG97850.026.75143438
TCAACGC135800.026.59164612
TCGTATG101500.026.06560335
GATCGGA1108550.025.828124
CAACGCA139350.025.76732413
AAGATCG1125800.025.5228752
AGATCGG1127400.025.4626673
ATCGGAA1127050.025.3988485
TCGGAAG1158600.024.726176
ATGCCGT108800.024.25997439
ATCAACG149250.024.23542611
CTCGTAT111950.023.54362734
CGGAAGA1228500.023.4135077
GACCGAT1210350.023.29821835
ACCGATC1211250.023.26891936
AGCGGTT1234000.023.22809413
AATGCCG1228900.023.20358827
GAGCGGT1234900.023.18043312
Produced by FastQC (version 0.11.2)