FastQCFastQC Report
Mon 1 Dec 2014
2014-01-29_02-27_03-10-BP5b_5987_TATT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-01-29_02-27_03-10-BP5b_5987_TATT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7792349
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG3856604.949213645333391Illumina Paired End PCR Primer 2 (100% over 43bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT94910.12179895946652286No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA91420.11732020729564346No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA421300.039.4317055
AAGATCG424550.039.2332233
ATCGGAA425250.039.070646
AGATCGG426150.039.034054
AGCGGTT423050.039.0331714
CGGTTCA422500.039.0237616
TAAGATC428300.038.931742
GAGCGGT424300.038.9132113
AATGCCG421000.038.8667428
GCGGTTC424550.038.85810515
AAGAGCG426400.038.835111
GACCGAT413550.038.76146336
ATAAGAT431250.038.735981
AGAGCGG427650.038.69771212
TCGGAAG429150.038.6876147
ACCGATC414550.038.6516437
GATCTCG418150.038.5640340
ATGCCGA424950.038.55254729
CGAGACC419300.038.4968233
CCGAGAC423850.038.4926432
Produced by FastQC (version 0.11.2)